Olympus Cell^R living cell real time long term fluorescence micro-imaging system.
Introduction:
Fluorescence was commonly used to label desired proteins or organelles and observed by fluorescence microscope. For fixed cell and tissue slide sample, we want to get a high quality XYZ image data. A qualified micro-image is relied on objective numerical aperture (NA), filter quality, light intensity, energy efficiency, CCD pixel size, QE, etc.
It is even more complex for living cell time lapse micro-imaging. Considering viability, pH value, cell proliferation, photo-toxicity and focusing accuracy, that is what OLYMPUS Cell^R provided. If extra devices are necessary for experiment, there are 7 TTL out/in connector to send/receive to/from devices during time lapse experiments.
With precise real-time controller, the Linux system combines all device in parallel for reducing jitter time from mini-sec to micro-sec. The time resolution and accuracy in Cell^R system is micro-sec, that means living cells suffer less photo-toxicity when acquiring fluorescence micro-image. Therefore we are able to observe very fast physiological response, like calcium influx, membrane potential change, etc. In this way, every image acquired during time lapse experiment is obtained precisely and exactly what happened at this moment.
Features:
Experimental Items:
Example Data:
DIC imaging: Endothelia cell Mitochondria dynamics
Ca2+ imaging: Thapsigargin (TG) stimulated Ca2+ jump
By Ming-Hsien Tsai from LSARC
FAQ/Contact:
Name:Chu, Chia-Ying
Tel No.:2371#23
E-mail:cychu@kmu.edu.tw